太阳成集团tyc9728-太阳集团见好就收9728

Location： Home» Faculty» Faculty

Faculty

Bin Xia

Ph.D

Professor, Peking University
Director and Chief Scientist, Beijing Nuclear Megnetic Resonance Center (BNMRC)
Address: Beijing NMR Center, Peking University, Beijing, 100871, China
Tel: +86-10-62758127 Fax: 010-62753790
email: binxia@pku.edu.cn

Research Group URL

Education

1989-1997 Ph.D in Biophysics University of Wisconsin-Madison, USA
1985-1989 B.S. in Biophysics/Physiology Peking University, China

Experience

2001- present
    Director and Chief Scientist Beijing NMR Center, China
    Cheung Kong Professor Peking University, China
    Doctoral Supervisor College of Chemistry and Molecular Engneering, Peking University
    Doctoral Supervisor School of Life Sciences, Peking University
1997-2001 Research Associate Department of Molecular Biology The Scripps Research Institute, USA

Research Areas

We are interested in studying 3D structures of proteins and their interactions with other proteins, nucleic acids, and small molecule ligands, using NMR spectroscopy along with other techniques in biochemistry and molecular biology, aiming to reveal protein structure-function relationship and molecular mechanisms for protein functions, including the roles of anti-tumor gene H-REV-107 family proteins in cell death, the structural basis of bacterial nucleoid-associated proteins in transcription regulation, the function of pro-apoptotic protein Bcl-XL in autophagy, the mechanism of glutaredoxins in iron and oxygen sensing, and protein folding and structural fluctuations using SARS-CoV main protease as a model.

Selected Publications

P Ding, K A McFarland, S Jin, G Tong, B Duan, A Yang, T R Hughes, J Liu, S L Dove, W W Navarre, B Xia et al: "A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT". PLoS pathogens 2015, 11(6):e1004967.
H Huang, H Wei, M Zou, X Xu, B Xia, F Liu, N Li: "Modulating fluorescence anisotropy of terminally labeled double-stranded DNA via the interaction between dye and nucleotides for rational design of DNA recognition based applications". Analytical chemistry 2015, 87(5):2748-2754.
H Jing, X Yang, J Zhang, X Liu, H Zheng, G Dong, J Nian, J Feng, B Xia*, Q Qian et al: "Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling". Nature communications 2015, 6:7395.
M Li, X Ma, Y H Chiang, K A Yadeta, P Ding, L Dong, Y Zhao, X Li, Y Yu, L Zhang, B Xia et al: "Proline isomerization of the immune receptor-interacting protein RIN4 by a cyclophilin inhibits effector-triggered immunity in Arabidopsis". Cell host & microbe 2014, 16(4):473-483.
L Lu, C Zhu, B Xia, C Yi: "Oxidative demethylation of DNA and RNA mediated by non-heme iron-dependent dioxygenases". Chemistry, an Asian journal 2014, 9(8):2018-2029.
Z Ming, W Wang, Y Xie, P Ding, Y Chen, D Jin, Y Sun, B Xia, L Yan, Z Lou: "Crystal structure of the novel di-nucleotide cyclase from Vibrio cholerae (DncV) responsible for synthesizing a hybrid cyclic GMP-AMP". Cell research 2014, 24(10):1270-1273.
L Wang, Y Li, J P Jacquot, N Rouhier, B Xia: "Characterization of poplar GrxS14 in different structural forms". Protein & cell 2014, 5(5):329-333.
H Wei, L Wang, X Ren, W Yu, J Lin, C Jin, B Xia: "Structural and functional characterization of tumor suppressors TIG3 and H-REV107". FEBS letters 2015, 589(11):1179-1186.
X Xu, T Li, Z Xu, H Wei, R Lin, B Xia, F Liu, N Li: "Automatic enumeration of gold nanomaterials at the single-particle level". Analytical chemistry 2015, 87(5):2576-2581.
L. Wang, Y. Li, J. P. Jacquot, N. Rouhier, B. Xia*. "Characterization of poplar GrxS14 in different structural forms".Protein Cell, 5, 329-33(2014).
H. Yao, S. Mi, W. Gong, J. Lin, N. Xu, S. Perrett, B. Xia, J. Wang, Y. Feng*. "Anti-apoptosis Proteins Mcl-1 and Bcl-xL Have Different p53-Binding Profiles".Biochemistry, 52(37):6324-34(2013).
B. Ouyang, L. Wang, S. Wan, Y. Luo, L. Wang, J. Lin, B. Xia*. "Solution structure of monomeric human FAM96A".J Biomol NMR, 56, 387-92(2013).
L. Chen, F. J. Xin, J. Wang, J. Hu, Y. Y. Zhang, S. Wan, L. S. Cao, C. Lu, P. Li, S. F. Yan, D. Neumann, U. Schlattner, B. Xia, Z. X. Wang, J. W. Wu*. "Conserved regulatory elements in AMPK".Nature, 498, E8-10(2013).
X. Xu, Y. Chen, H. Wei, B. Xia, F. Liu, N. Li*. "Counting bacteria using functionalized gold nanoparticles as the light-scattering reporter".Anal Chem, 84, 9721-8(2012).
X. Kang, N. Zhong, P. Zou, S. Zhang, C. Jin, B. Xia*. "Foldon unfolding mediates the interconversion between Mpro-C monomer and 3D domain-swapped dimer".Proc Natl Acad Sci U S A, 84, 9721-8(2012).
L. Wang, B. Ouyang, Y. Li, Y. Feng, J. P. Jacquot, N. Rouhier, B. Xia*. "Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin".Protein Cell, 3, 714-21(2012).
C. Zhou, J. Zhou, F. Sheng, H. Zhu, X. Deng*, B. Xia, J. Lin*. "The heme oxygenase-1 inhibitor ZnPPIX induces non-canonical, Beclin 1-independent, autophagy through p38 MAPK pathway".Acta Biochim Biophys Sin (Shanghai), (2012).
K. Zhang, Z. Wang, X. Liu, C. Yin, Z. Basit, B. Xia*, W. Liu*. "Dissection of influenza A virus M1 protein: pH-dependent oligomerization of N-terminal domain and dimerization of C-terminal domain".PLoS One, 7, e37786(2012).
C. Zhou, W. Zhong, J. Zhou, F. Sheng, Z. Fang, Y. Wei, Y. Chen, X. Deng*, B. Xia, J. Lin*. "Monitoring autophagic flux by an improved tandem fluorescent-tagged LC3 (mTagRFP-mWasabi-LC3) reveals that high-dose rapamycin impairs autophagic flux in cancer cells".Autophagy, 8(2012).
S. S. Ali, B. Xia, J. Liu, W. W. Navarre*. "Silencing of foreign DNA in bacteria".Curr Opin Microbiol, 15, 175-81(2012).
L. Wang, W. Yu, X. Ren, J. Lin, C. Jin, B. Xia*. "1H, 13C, and 15N resonance assignments of the N-terminal domain of human TIG3".Biomol NMR Assign, (2012)
B. R.G. Gordon, Y Li, A. Coteb, M. T. Weirauch, P. Ding, T. R. Hughes, W. W. Navarre, B. Xia*, and J. Liu* “Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins” Proc. Natl. Acad. Sci. in press (2011)
B. Xia* and X. Kang “Activation and Maturation of SARS-CoV Main Protease”, Protein Cell 2: 282-90 (2011).
W. Gong, J. Wang, Z. Chen, B. Xia*, and G. Lu* “Solution Structure of LCI, a Novel Antimicrobial Peptide from Bacillus subtilis”, Biochemistry 50: 3621-7 (2011).
L. Wang, X. Ren, Y. Li, N. Rouhier, J. P. Jacquot, C. Jin, B. Xia* "1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula x tremuloides".Biomol NMR Assign, 5, 121-4(2011).
F. Song, X. Zhang, X. Ren, P. Zhu, J. Xu, L. Wang, Y. Li, N. Zhong, Q. Ru, D. Zhang, J. Jiang, B. Xia*, & Z. Chen* “Cyclophilin A (CyPA) induces chemotaxis independent of its peptidylprolyl Cis-trans isomerase activity: Direct binding between CyPA and the ectodomain of CD147”, J. Biol. Chem. 286: 8197-203 (2011).
J. Zhou, J. Lin, C. Zhou, X. Deng*, & B. Xia* “Cytotoxicity of Red Fluorescent Protein DsRed is Associated with the Suppression of Bcl-xL Translation”, FEBS Lett. 585: 821-7 (2011).
J. Zhou, J. Lin, C. Zhou, X. Deng*, & B. Xia* “An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein” Acta Biochim. Biophys. Sin. 43: 239-44 (2011).
S. Zhang, N. Zhong, F. Xue, X. Kang, X. Ren, J. Chen, C. Jin, Z. Lou* and B. Xia* “3D domain swapping as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease”, Protein Cell1: 371–83 (2010).
S. Zhang, N. Zhong, X. Ren, C. Jin, B. Xia* “1H, 13C and 15N resonance assignments of SARS-CoV main protease N-terminal domain”.Biomol NMR Assign (2010).
S. Tian, J. Lin, J. Zhou, X. Wan, Y. Li, X. Ren, W. Yu, W. Zhong, J. Xiao, F. Sheng, Y. Chen, C. Jin, S. Li, Z. Zheng*, and B. Xia* “Beclin 1-independent autophagy induced by a Bcl-XL/Bcl-2 targeting compound Z18”,Autophagy 6: 1032-41 (2010).
X. Ren, J. Lin*, C. Jin, and B. Xia* “Solution structure of the N-terminal catalytic domain of human H-REV107 - a novel circular permutated NlpC/P60 domain”, FEBS Lett. 584:4222-6 (2010).
J. Hu, D. Li, X.-D. Su, C. Jin, and B. Xia* “Solution Structure and Conformational Heterogeneity of Acylphosphatase from Bacillus subtilis”, FEBS Lett. 584: 2852-2856 (2010).
J. Lin, N. Wang, Y. Li, Z. Liu, S. Tian, L. Zhao, Y. Zheng, S. Liu, S. Li, C. Jin, and B. Xia* “LEC-BiFC: A New Method for Rapid Assay of Protein Interaction”, Biotech. Histochem. DOI: 10.3109/10520295.2010.483068 (2010).
X. Ren, J. Lin, C. Jin, and B. Xia* “1H, 13C and 15N resonance assignments of human H-REV107 N-terminal domain”, Biomol. NMR Assign. 4: 175-8 (2010).
B. R.G. Gordon, Y. Li , L. Wang , A. Sintsova , H. V. Bakel , S. Tian, W. W. Navarre, B. Xia*, and J. Liu* “Lsr2 is A Nucleoid-Associated Protein that Targets AT-Rich Sequences and Virulence Genes in Mycobacterium tuberculosis”, Proc. Natl. Acad. Sci. 107: 5154-5159 (2010).
J. Lin, Z. Zheng, Y. Li, W. Yu, W. Zhong, S. Tian, F. Zhao, X. Ren, J. Xiao, N. Wang, S. Liu, L. Wang, F. Sheng, Y. Chen, C. Jin, S. Li*, and B. Xia* “A Novel Bcl-XL Inhibitor Z36 that Induces Autophagic Cell Death in Hela Cells”, Autophagy 5: 314-320 (2009).
J. Hu, G. Cui, C. Li, C. Liu, E. Shang, L. Lai, C. Jin, J. Wang*, and B. Xia* “Structure and Novel Functional Mechanism of Drosophila SNF in Sex-lethal Splicing”, PloS ONE4: e6890 (2009).
N. Zhong, S. Zhang, F. Xue, X. Kang, P. Zou, J. Chen, C. Liang, Z. Rao, C. Jin, Z. Lou*, and B. Xia* “C-Terminal Domain of SARS-CoV Main Protease Can Form a 3D Domain-Swapped Dimer” Protein Sci. 18: 839-44 (2009).
T. Wang, K. Shao, Q. Chu, Y. Ren, Y. Mu, L. Qu, J. He, C. Jin, and B. Xia* “Automics: An Integrated Platform for NMR-Based Metabonomics Spectral Processing and Data Analysis”,　BMC Bioinformatics 10: 83 (2009).
Ren, T. Wang, Y. Peng, B. Xia*, L. J. Qu*. "Distinguishing transgenic from non-transgenic Arabidopsis plants by (1)H NMR-based metabolic fingerprinting".J Genet Genomics, 36, 621-8(2009).
W. Yu, J. Lin, C. Jin, and B. Xia* “Solution Structure of Human Zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats” J. Mol. Biol. 386: 903-912 (2009).
W. Yu, J. Hu, B. Yu, W. Xia, C. Jin, and B. Xia* “Solution Structure of GSP13 From Bacillus subtilis Exhibits an S1 Domain Related to Cold Shock Proteins”, J. Biomol. NMR. 43: 255-259 (2009).
W. Gong, Y. Li, G. Cui, J. Hu, H. Fang, C. Jin, and B. Xia* “Solution Structure and Catalytic Mechanism of Human Protein Histidine Phosphatase 1”,　Biochem. J. 418: 337-344 (2009).
W. Yu, B. Yu, J. Hu, W. Xia, C. Jin, and B. Xia* “1H, 13C, and 15N Resonance Assignments of a General Stress Protein GSP13 from Bacillus subtilis”, Biomol. NMR Asign. 2: 163–165 (2008).
N. Zhong, S. Zhang, P. Zou, J. Chen, X. Kang, Z. Li, C. Liang, C. Jin, and B. Xia* “Without its N-Finger, SARS-CoV Main Protease can Form a Novel Dimer through its C-Terminal Domain”, J. Virol. 82: 4227-4234, (2008).
W. Yu, C. Jin, and B. Xia* “1H, 13C, and 15N Resonance Assignments of Human ζ-COP”, Biomol. NMR Asign.2: 73–75 (2008).
W. Gong, G. Cui, C. Jin, and B. Xia* “1H, 13C and 15N Resonance Assignments of Human Phosphohistidine Phosphatase 1 (PHPT1)”, Biomol. NMR Asign. 1: 229-231 (2007).
Y. Feng, N. Zhong, N. Rouhier, T. Hase, M. Kusunoki, J.-P. Jacquot, C. Jin, and B. Xia* “Structural Insight into Poplar Glutaredoxin C1 with a Bridging Iron-Sulfur Cluster At the Active Site”, Biochemistry, 45, 7998-8008 (2006).
G. Cui, B. Nan, J. Hu, Y. Wang, C. Jin, and B. Xia* “Identification and Solution Structures of a Single-Domain Biotin/Lipoyl Attachment Protein from Bacillus subtilis”, J. Biol. Chem. 281: 20598-20607 (2006).
Y. Feng, N. Rouhier, J.-P. Jacquot, and B. Xia* “1H, 15N, and 13C Resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides”, J. Biomol. NMR 31: 263-264 (2005).
N. Zhong, Q. Huang, C. Jin, and B. Xia* “1H, 13C and 15N resonance assignments of the N-terminal Domain of the SARS CoV Nucleocapsid Protein”, 31: 79-80 (2005).