Research Experience
2021–Present: Research Associate Professor, College of Chemistry and Molecular Engineering, Peking University
2020–2021: Senior Research Scientist，NuProbe USA. Inc.
2018–2020: Postdoctoral Scholar, Rice University, Advisor: Prof. David Y. Zhang
2013-2018:Ph.D in Chemistry, Massachusetts institute of technology, Advisor: Prof. Bradley L. Pentelute.
2009-2013：B.S. in Chemistry, Peking University, Advisor: Prof. Xing Chen
Research Interests
All cells are covered with a dense layer of structurally complex and dynamically regulated glycans. Towards the goal of deciphering the glycocode, we are interested in developing novel chemical glycobiology tools and integrating multidisciplinary technologies to understand the structure-property relationship of glycans.
We focus on the engineering of glycosyltransferases and glycosidases of bacteria origin to develop specific glycan labeling and editing tools. The ability to achieve affinity labeling of glycans in a highly specific and multiplexed manner is the fundamental requirement to translate complex glycan information into amplifiable DNA barcodes for further single-cell and in-situ glycan analysis. In addition to developing such labeling toolbox, we are particularly interested in single-cell multimodal analysis of cell surface glycan and the expression level of glycan-related genes to investigate how glycans are determined and dynamically regulated. By Abnormal glycosylation in disease， clinical-oriented chemical biology research.
Selected Publications
(#co-first authorship, *co-corresponding authorship)
- Liu, W.#; Tang, Q.#; Meng, L.#; Hu, S.; Sun, D.; Li, S.; Dai, P.*; Chen, X.* Interbacterial Chemical Communication-Triggered Nascent Proteomics. Angew. Chem. Int. Ed. 2023, 62(5): e202214010.
- Sun, J.#; Huang, Z.#; Du, Y.#; Lv, P.; Fan, X.; Dai, P.*; Chen, X.* Metabolic Glycan Labeling in Primary Neurons Enabled by Unnatural Sugars with No S‐Glyco- Modification, ACS Chem. Bio. 2023, 18(6): 1416-1424
- Dai, P.#, Wu, L. R.#, Chen, S. X., Wang, M. X. and Cheng, L. Y., Zhang, J. X., Hao, P., Yao, W. Zarka, J. and Issa, G. C., Kwong, L. and Zhang, D. Y. Calibration-free NGS quantitation of mutations below 0.01% VAF. Nat. Commun. 2021, 12, 6123.
- Wu, L. R.#, Dai, P.#, Wang, M. X., Chen, S.X., Cohen, E. N., Jayachandran, J., Zhang, J. X., Serrano, A. V., Xie, N. G., Ueno N. T., Reuben, J. M., Barcenas, C. H.*, and Zhang, D. Y.* Ensemble of Nucleic Acid Absolute Quantitation Modules for Copy Number Variation Detection and RNA Profiling. Nat. Commun. 2022,13,1791
- Kubota, K.#, Dai, P.#, Pentelute, B. L.*, & Buchwald, S. L.* Palladium oxidative addition complexes for peptide and protein cross-linking. J. Am. Chem. Soc. 2018, 140(8), 3128-3133.
- Zhang, C.#, Dai, P.#, Vinogradov, A. A., Gates, Z. P., & Pentelute, B. L. Site-Selective Cysteine-Cyclooctyne Conjugation. Angew. Chem. Int. Ed., 2018, 57(22), 6459-6463.
- Dai, P.; Williams, J. K.; Zhang, C.; Welborn, M.; Shepherd, J. J.; Zhu, T.; Van Voorhis, T.; Hong, M.; Pentelute, B. L. A structural and mechanistic study of π-clamp-mediated cysteine perfluoroarylation. Scientific Reports. 2017, 7 (1), 7954.
- Dai, P.; Zhang, C.; Welborn, M.; Shepherd, J. J.; Zhu, T.; Van Voorhis, T.; Pentelute, B. L. Salt Effect Accelerates Site-Selective Cysteine Bioconjugation. ACS Cent. Sci. 2016, 2, 637–646.
